4 more days till a new semester begins. Yes, I stayed back in INTI this semester break...did not go back to Brunei though my bro went back for like less than a week? -.-" crazy guy. Surprisingly...this semester break wasn't boring at all!! Thought that the week after camp will be boring but I rarely have time to myself! Wanted a movie marathon but it never happened! Haven't settle my university application >.<"
Last week before I went for camp, had supper with my sir...and he said that FAS (Faculty of Science) needed students to be 'models' to pose in the lab. Guess its for INTI's biotech program or Australia Degree Transfer Science. And so far he knows I am the only one who is going to stay back for the semester break...so I agreed. Got an e-mail from Dr.Choong, a cute lecturer (he is short XD). During my A-levels practical examination, my burette keep leaking!! So he changed it for me TWICE! That's how I remember him! And when I told him, he was surprised because he said that I look familiar to him :P
Had our photo shoot on Tuesday, my seniors and I. They came all the way from KL o.O FYI, we are NOT paid to be 'models' in this photo shoot. Its voluntary. And I think that the photographer was paid by the hour so he took his own sweet time adjusting the light here and there -.-" We were suppose to pose for 9 experiments, but we managed to pose for half of them! #$@#%@
We were suppose to pose for...
1. Students using gel doc
2. Students performing chromatography with fraction collector
3. Students looking through a microscope (its not a normal microscope, its a huge electron microscope! I WAS SUPPOSE TO HAVE AN INDIVIDUAL POSE FOR THIS BUT NOT ENOUGH TIME!)
4. Student performing plant tissue culture
5. Student loading horizontal gel electrophoresis
6. Student loading vertical gel electrophoresis
7. Students counting corn phenotype
8. Students inspecting blue-white colonies under a lamp
9. Students inspecting plant tissue culture
Last week before I went for camp, had supper with my sir...and he said that FAS (Faculty of Science) needed students to be 'models' to pose in the lab. Guess its for INTI's biotech program or Australia Degree Transfer Science. And so far he knows I am the only one who is going to stay back for the semester break...so I agreed. Got an e-mail from Dr.Choong, a cute lecturer (he is short XD). During my A-levels practical examination, my burette keep leaking!! So he changed it for me TWICE! That's how I remember him! And when I told him, he was surprised because he said that I look familiar to him :P
Had our photo shoot on Tuesday, my seniors and I. They came all the way from KL o.O FYI, we are NOT paid to be 'models' in this photo shoot. Its voluntary. And I think that the photographer was paid by the hour so he took his own sweet time adjusting the light here and there -.-" We were suppose to pose for 9 experiments, but we managed to pose for half of them! #$@#%@
We were suppose to pose for...
1. Students using gel doc
2. Students performing chromatography with fraction collector
3. Students looking through a microscope (its not a normal microscope, its a huge electron microscope! I WAS SUPPOSE TO HAVE AN INDIVIDUAL POSE FOR THIS BUT NOT ENOUGH TIME!)
4. Student performing plant tissue culture
5. Student loading horizontal gel electrophoresis
6. Student loading vertical gel electrophoresis
7. Students counting corn phenotype
8. Students inspecting blue-white colonies under a lamp
9. Students inspecting plant tissue culture
Hmm...so we posed for the gel doc, chromatography with fraction collector,plant tissue culture and horizontal gel electrophoresis. I posed for the chromatography with fraction collector with a senior and plant tissue culture with 2 others.
this is the blue-white colony. White color isn't that visible here. Used to detect if its a recombinant DNA or a non-recombinant DNA.
This is a genetically modified corn which was dried up. 
You can see some purple color there too. Unique eh? Imagine counting those -.-
Ahh this is the huge horizontal gel electrophoresis that took up most of the time! A dude did it, not me :). He had to pose when he takes up the sample, and another loading it...
Plant tissue culture! This is the sample of a successfully grown plantlet. Takes a long time to grow! And this year was the first time in INTI's history that it was successful *clap clap*
Ah hah! Plant tissue culture! Once the plantlets were transferred
The chromatography with fraction collector! The thing on the right is where we put the tubes, and we can set how many drops we want per tube and it will move to the next tube once the amount is obtained. Cool thing ;) That is how we obtain the color we want 
We have no idea why a green solution was obtained...it should be yellow!
This is something we have not seen before in real life (only in the notes), a vertical gel electrophoresis! The gel is actually in between the 2 layers of glass, one thicker layer and another thin fragile glass. Its not easy removing the gel. The gel is located one at each end.Marketing office did not pay us, nor did INTI. But we had to fill in an agreement form to allow our photos to be used. So Dr.Choong said that we shall not 'model' with our formal clothes and the other lab. Its a waste of time. Hope I am able to get the photos next time :D
P.S if you do recognize me in the newspaper or any media, do let me know!! Am not sure which picture will they be using and where
P.S if you do recognize me in the newspaper or any media, do let me know!! Am not sure which picture will they be using and where
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Dr Choong took us out to Ocean Village for lunch (out from his own pocket) and a particular lady working there is RUDE!! We refilled our pot of tea twice, and we wanted another refill after our meal. She said "no hot water". WTF?! HOW CAN A RESTAURANT NOT HAVE HOT WATER? So we took our own sweet time chatting then she gave us her moody face and moody tone and asked us to pay the bill because they wanna close the store. WTH?! How can they chase away customers! They usually close at 2.30pm and its only 3pm. Geeez. Went back to help clean up the lab and talked with him for hours! @.@ Dr.Choong wanted to make us of my friend and I, so he offered to teach us something on Thursday. But sadly, my friend had fever...so he can't make it.
I did...PLANT TISSUE CULTURE!! Worked on a blue African Violet. First time doing it, and it was great!! 1 secret for a plant tissue culture to be successful...no contamination! The lamniar flow is the most important part! Went to the lab early just to set up the laminar flow. Had to clean it with 70% ethanol at both sides and the surface. Closed it, and left the UV on while I went for lunch.
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Dr Choong took us out to Ocean Village for lunch (out from his own pocket) and a particular lady working there is RUDE!! We refilled our pot of tea twice, and we wanted another refill after our meal. She said "no hot water". WTF?! HOW CAN A RESTAURANT NOT HAVE HOT WATER? So we took our own sweet time chatting then she gave us her moody face and moody tone and asked us to pay the bill because they wanna close the store. WTH?! How can they chase away customers! They usually close at 2.30pm and its only 3pm. Geeez. Went back to help clean up the lab and talked with him for hours! @.@ Dr.Choong wanted to make us of my friend and I, so he offered to teach us something on Thursday. But sadly, my friend had fever...so he can't make it.
I did...PLANT TISSUE CULTURE!! Worked on a blue African Violet. First time doing it, and it was great!! 1 secret for a plant tissue culture to be successful...no contamination! The lamniar flow is the most important part! Went to the lab early just to set up the laminar flow. Had to clean it with 70% ethanol at both sides and the surface. Closed it, and left the UV on while I went for lunch.
That is Dr.Choong. He looks better real life than here...seriously XD. Right infront of him is the laminar flow. He worked on one, I worked on the other. Equipments are to be sterilized at ALL times! As long as it is out of the laminar flow, and before putting it into the laminar flow. Wipe them with ethanol! Had to wipe the bunser burner too! And use the Aseptic technique that involves slight burning to the equipment to kill the remaining microorganisms. Even the plastic container at each side and the cover! Hands are to be washed with ethanol before handling anything. There are certain ways to follow to transfer the plantlets in and out of the container to the petri dish! Just ensure that there is no crossing over, and do not use your dirty hands to touch the petri dish!
I did 3 samples of plant tissue culture. The plantlets are still small though, and Im suppose to cut up the big plantlets from its bottom shoot. O well ><. And they are to be put upright in the gel, which I didn't in some... This is part of Biotechnology. Guess he wanted us to try and see how we feel about it. Well, he said it was my first time and I cut the plantlets really nicely :D. And first impression, he said I looked like a Biomed student T.T why...
Had a great experience working on this, don't be jealous! You'll get your chance someday ;P
Had a great experience working on this, don't be jealous! You'll get your chance someday ;P
